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AKT2 monoclonal antibody, clone 4H7

  • Catalog # : MAB13976
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant human AKT2.
  • Immunogen:
  • Recombinant protein corresponding to full length human AKT2.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse, Primates, Rat
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ChIP (1 - 3 uL)
    Immunocytochemistry (1:10 - 1:100)
    Immunofluorescence (1:10 - 1:100)
    Immunohistochemistry (1:200)
    Immunoprecipitation (2 ug)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.1% BSA, 30% glycerol, 0.05% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: MCF-7, Lane 2: HeLa, Lane 3: HepG2, Lane 4: A549, Lane 5: HEK 293T, Lane 6: Jurkat, Lane 7: A-431, Lane 8: U-2 OS, Lane 9: COS-7, Lane 10: NIH/3T3, and Lane 11: NRK cells with AKT2 monoclonal antibody, clone 4H7 (Cat # MAB13976).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human medulla oblongata (right) and human esophageal cancer tissues (left) with AKT2 monoclonal antibody, clone 4H7 (Cat # MAB13976).
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemical staining of mouse C2C12 cell with AKT2 monoclonal antibody, clone 4H7 (Cat # MAB13976) showing staining in the cytoplasm and nucleus (right) compared to a negative control without primary antibody (left).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of HeLa cell with AKT2 monoclonal antibody, clone 4H7 (Cat # MAB13976) showing staining in the cytoplasm and nucleus (right) compared to a negative control without primary antibody (left).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of MCF-7 cell with AKT2 monoclonal antibody, clone 4H7 (Cat # MAB13976) showing staining in the cytoplasm and nucleus (right) compared to a negative control without primary antibody (left).
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation analysis of HeLa cell with AKT2 monoclonal antibody, clone 4H7 (Cat # MAB13976) at 1:1000 dilution.
  • Application Image
  • ChIP
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 208
  • Gene Name:
  • AKT2
  • Gene Alias:
  • PKBB,PKBBETA,PRKBB,RAC-BETA
  • Gene Description:
  • v-akt murine thymoma viral oncogene homolog 2
  • Gene Summary:
  • This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq
  • Other Designations:
  • Murine thymoma viral (v-akt) homolog-2,rac protein kinase beta
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