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ACO2 monoclonal antibody, clone 7G4

  • Catalog # : MAB13974
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant human ACO2.
  • Immunogen:
  • Recombinant protein corresponding to full length human ACO2.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse, Primates, Rat
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Immunocytochemistry (1:100)
    Immunofluorescence (1:100)
    Immunohistochemistry (1:800)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.1% BSA, 30% glycerol, 0.05% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: HEK 293T, Lane 2: A-549, Lane 3: Jurkat, Lane 4: A-431, Lane 5: MCF-7, Lane 6: HeLa, Lane 7: U-2 OS, Lane 8: K-562, Lane 9: COS-7, Lane 10: 3T3-L1, and Lane 11: NRK cells with ACO2 monoclonal antibody, clone 7G4 (Cat # MAB13974).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon carcinoma, myocardial carcinoma, hepatic carcinoma and renal cell carcinoma tissues with ACO2 monoclonal antibody, clone 7G4 (Cat # MAB13974).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of HeLa cell with ACO2 monoclonal antibody, clone 7G4 (Cat # MAB13974) and the cell nuclei were stained with Hoechst 33342 dye (blue).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of NIH/3T3 cell with ACO2 monoclonal antibody, clone 7G4 (Cat # MAB13974) and the cell nuclei were stained with Hoechst 33342 dye (both panels, blue).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Gene Information
  • Entrez GeneID:
  • 50
  • Gene Name:
  • ACO2
  • Gene Alias:
  • ACONM,MGC20605,MGC33908
  • Gene Description:
  • aconitase 2, mitochondrial
  • Gene Summary:
  • The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000042146,OTTHUMP00000165920,aconitase 2,aconitate hydratase,citrate hydro-lyase
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