Nos3 monoclonal antibody, clone M221
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of human umbilical vein endothelial cells before (Lane 1) and after (Lane 2) treatment with lambda phosphatase. The blots were probed with Nos3 monoclonal antibody, clone M221 (Cat # MAB1390).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant Nos3.
Immunogen
Recombinant protein corresponding to C terminal of mouse Nos3.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This sequence is conserved in human and rat eNOS, and has low homology to other NOS family members.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of human umbilical vein endothelial cells before (Lane 1) and after (Lane 2) treatment with lambda phosphatase. The blots were probed with Nos3 monoclonal antibody, clone M221 (Cat # MAB1390).Enzyme-linked Immunoabsorbent Assay
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Gene Info — Nos3
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Interactome
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Publication Reference
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Regulation of endothelial and myocardial NO synthesis by multi-site eNOS phosphorylation.
Mount PF, Kemp BE, Power DA.
Journal of Molecular and Cellular Cardiology 2007 Feb; 42(2):271.
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Inactivation of phosphorylated endothelial nitric oxide synthase (Ser-1177) by O-GlcNAc in diabetes-associated erectile dysfunction.
Musicki B, Kramer MF, Becker RE, Burnett AL.
PNAS 2005 Aug; 102(33):11870.
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Cloning and characterization of inducible nitric oxide synthase from mouse macrophages.
Xie QW, Cho HJ, Calaycay J, Mumford RA, Swiderek KM, Lee TD, Ding A, Troso T, Nathan C.
Science 1992 Apr; 256(5054):225.
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Regulation of endothelial and myocardial NO synthesis by multi-site eNOS phosphorylation.
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