Prkcq monoclonal antibody, clone M217
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Prkcq in human Jurkat cell lysate. The blot was probed with Prkcq monoclonal antibody, clone M217 (Cat # MAB1389) at 1:250 (lane 1) and 1:1000 (lane 2).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant Prkcq.
Immunogen
Recombinant protein corresponding to N-terminus of mouse Prkcq.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This sequence is conserved in human and rat PKCq, and has low homology to other PKC family members.
Form
Liquid
Isotype
IgG2a
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Prkcq in human Jurkat cell lysate. The blot was probed with Prkcq monoclonal antibody, clone M217 (Cat # MAB1389) at 1:250 (lane 1) and 1:1000 (lane 2).Enzyme-linked Immunoabsorbent Assay
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Gene Info — Prkcq
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Publication Reference
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A Ras activation pathway dependent on Syk phosphorylation of protein kinase C.
Kawakami Y, Kitaura J, Yao L, McHenry RW, Kawakami Y, Newton AC, Kang S, Kato RM, Leitges M, Rawlings DJ, Kawakami T.
PNAS 2003 Aug; 100(16):9470.
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The molecular heterogeneity of protein kinase C and its implications for cellular regulation.
Nishizuka Y.
Nature 1988 Aug; 334(6184):661.
Application：IF, IHC, Rat, Rat cerebellar cortex.
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A Ras activation pathway dependent on Syk phosphorylation of protein kinase C.
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