Cdh1 monoclonal antibody, clone M168
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot of human A-431 cells treated with pervanadate (1 mM) for 30 min (lane 1) then treated with akaline phosphatase (lane 2). Blots were probed with Cdh1 monoclonal antibody, clone M168 (Cat # MAB1388).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant Cdh1.
Immunogen
Recombinant protein corresponding to C-terminus of mouse Cdh1.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This sequence is highly conserved in human and rat E-cadherin.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:2000)
Immunocytochemistry (1:250)
Immunoprecipitation (1:100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot of human A-431 cells treated with pervanadate (1 mM) for 30 min (lane 1) then treated with akaline phosphatase (lane 2). Blots were probed with Cdh1 monoclonal antibody, clone M168 (Cat # MAB1388).Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — Cdh1
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Publication Reference
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Involvement of Src family kinases in N-cadherin phosphorylation and beta-catenin dissociation during transendothelial migration of melanoma cells.
Qi J, Wang J, Romanyuk O, Siu CH.
Molecular Biology of the Cell 2005 Dec; 17(3):1261.
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Interaction of the adaptor protein Shc and the adhesion molecule cadherin.
Xu Y, Guo DF, Davidson M, Inagami T, Carpenter G.
The Journal of Biological Chemistry 1997 May; 272(21):13463.
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The cadherins: cell-cell adhesion molecules controlling animal morphogenesis.
Takeichi M.
Development 1988 Apr; 102(4):639.
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Involvement of Src family kinases in N-cadherin phosphorylation and beta-catenin dissociation during transendothelial migration of melanoma cells.
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