Brain tubulin monoclonal antibody, clone M154
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of purified human brain tubulin untreated (lane 1) or treated with ERK2 kinase to phosphorylate Ser-172 (lane 2). The blot was probed with Brain tubulin monoclonal antibody, clone M154 (Cat # MAB1387).
Immunofluorescence
Immunocytochemical labeling in C2C12 cells using Brain tubulin monoclonal antibody, clone M154 (Cat # MAB1387).
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Specification
Product Description
Mouse monoclonal antibody raised against full length native brain tubulin.
Immunogen
Native purified tubulin from porcine brain.
Host
Mouse
Reactivity
Human, Mouse, Pig, Rat
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Native Purifid Protein.
Recommend Usage
ELISA (1:2000)
Immunocytochemistry (1:200)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of purified human brain tubulin untreated (lane 1) or treated with ERK2 kinase to phosphorylate Ser-172 (lane 2). The blot was probed with Brain tubulin monoclonal antibody, clone M154 (Cat # MAB1387).Immunocytochemistry
Immunofluorescence
Immunocytochemical labeling in C2C12 cells using Brain tubulin monoclonal antibody, clone M154 (Cat # MAB1387).Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Microtubule regulation in mitosis: tubulin phosphorylation by the cyclin-dependent kinase Cdk1.
Fourest-Lieuvin A, Peris L, Gache V, Garcia-Saez I, Juillan-Binard C, Lantez V, Job D.
Molecular Biology of the Cell 2005 Dec; 17(3):1041.
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Post-translational modifications regulate microtubule function.
Westermann S, Weber K.
Nature Reviews. Molecular Cell Biology 2003 Dec; 4(12):938.
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Expression of unphosphorylated class III beta-tubulin isotype in neuroepithelial cells demonstrates neuroblast commitment and differentiation.
Fanarraga ML, Avila J, Zabala JC.
The European Journal of Neuroscience 1999 Feb; 11(2):517.
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Microtubule regulation in mitosis: tubulin phosphorylation by the cyclin-dependent kinase Cdk1.
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