Bcar1 monoclonal antibody, clone M144
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant Bcar1.
Immunogen
Recombinant protein corresponding to C-terminus of rat Bcar1.
Host
Mouse
Reactivity
Chicken, Human, Mouse, Rat
Specificity
This rat sequence is highly conserved in human, mouse, and chicken p130 Cas.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:2000)
Immunocytochemistry (1:250)
Immunoprecipitation (5-10 ul)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — Bcar1
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Publication Reference
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Subsets of the major tyrosine phosphorylation sites in Crk-associated substrate (CAS) are sufficient to promote cell migration.
Shin NY, Dise RS, Schneider-Mergener J, Ritchie MD, Kilkenny DM, Hanks SK.
The Journal of Biological Chemistry 2004 Sep; 279(37):38331.
Application：WB, Mouse, MEFs.
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Phosphorylation-dependent cleavage of p130cas in apoptotic rat-1 cells.
Hoon Kim D, Jeon Choi S, Kook S, Kim W, Keun Song W.
Biochemical and Biophysical Research Communications 2003 Jan; 300(1):141.
Application：WB-Ce, Rat, Rat-1 cells.
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Processive phosphorylation of p130Cas by Src depends on SH3-polyproline interactions.
Pellicena P, Miller WT.
J Biol Chem 2001 Jun; 276(30):28190.
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Subsets of the major tyrosine phosphorylation sites in Crk-associated substrate (CAS) are sufficient to promote cell migration.
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