Mapk14 monoclonal antibody, clone M138
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of A-431 cells serum starved overnight (lane 1) or treated with pervanadate (1 mM) for 30 minutes (lane 2). The blot was probed with Mapk14 monoclonal antibody, clone M138 (Cat # MAB1384).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant Mapk14.
Immunogen
Recombinant protein corresponding to C-terminus of rat Mapk14.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This sequence is identical in human and mouse p38a.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:2000)
Immunocytochemistry (1:500)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of A-431 cells serum starved overnight (lane 1) or treated with pervanadate (1 mM) for 30 minutes (lane 2). The blot was probed with Mapk14 monoclonal antibody, clone M138 (Cat # MAB1384).Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — Mapk14
Entrez GeneID
81649Gene Name
Mapk14
Gene Alias
CSBP, CSPB1, Csbp1, Csbp2, Exip, Hog, MGC105413, Mxi2, Prkm14, Prkm15, RK, Sapk2A, p38, p38Hog, p38alpha
Gene Description
mitogen activated protein kinase 14
Gene Ontology
HyperlinkOther Designations
Csaids binding protein|MAP kinase 2|MAP kinase Mxi2|MAX-interacting protein 2|cytokine suppressive anti-inflammatory drug binding protein|cytokine-supressive anti-inflammatory drug binding protein|mitogen-activated protein kinase 14|p38 MAP kinase|p38 mit
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Publication Reference
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Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine.
Raingeaud J, Gupta S, Rogers JS, Dickens M, Han J, Ulevitch RJ, Davis RJ.
The Journal of Biological Chemistry 1995 Mar; 270(13):7420.
Application：IF, IP, WB-Ce, WB-Tr, Human, Monkey, COS, HeLa cells.
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Interleukin-1 activates a novel protein kinase cascade that results in the phosphorylation of Hsp27.
Freshney NW, Rawlinson L, Guesdon F, Jones E, Cowley S, Hsuan J, Saklatvala J.
Cell 1994 Sep; 78(6):1039.
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A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins.
Rouse J, Cohen P, Trigon S, Morange M, Alonso-Llamazares A, Zamanillo D, Hunt T, Nebreda AR.
Cell 1994 Sep; 78(6):1027.
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Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine.
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