Jup monoclonal antibody, clone M111
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More Files
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Immunoprecipitation
Western blot analysis of Jup immunoprecipitated from lysates prepared from A-431 cells treated with pervanadate. The immunoprecipitation was performed using mouse Jup monoclonal antibody. The blots were probed with Jup monoclonal antibody, clone M111 (Cat # MAB1383). The immunoprecipitated Jup was untreated (lane 1) or treated with alkaline phosphatase (lane 2) then probed with the antibodies.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant Jup.
Immunogen
Recombinant protein corresponding to C-terminus of rat Jup.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This peptide sequence is highly conserved in human and mouse g-Catenin.
Form
Liquid
Isotype
IgG2a
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:2000)
Immunoprecipitation (5-10 ul)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunoprecipitation
Western blot analysis of Jup immunoprecipitated from lysates prepared from A-431 cells treated with pervanadate. The immunoprecipitation was performed using mouse Jup monoclonal antibody. The blots were probed with Jup monoclonal antibody, clone M111 (Cat # MAB1383). The immunoprecipitated Jup was untreated (lane 1) or treated with alkaline phosphatase (lane 2) then probed with the antibodies.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Jup
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Publication Reference
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Tyrosine phosphorylation of plakoglobin causes contrary effects on its association with desmosomes and adherens junction components and modulates beta-catenin-mediated transcription.
Miravet S, Piedra J, Castano J, Raurell I, Franci C, Dunach M, Garcia de Herreros A.
Molecular and Cellular Biology 2003 Oct; 23(20):7391.
Application：IF, IP-WB, WB-Ce, WB-Tr, Human, Rat, SW480, RWP1, IEC18 cells.
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A homolog of the armadillo protein in Drosophila (plakoglobin) associated with E-cadherin.
McCrea PD, Turck CW, Gumbiner B.
Science 1991 Nov; 254(5036):1356.
Application：WB-Ce, Dog, Frog, A6, MDCK cells.
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Tyrosine phosphorylation of plakoglobin causes contrary effects on its association with desmosomes and adherens junction components and modulates beta-catenin-mediated transcription.
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