STAT1 (phospho Y701) monoclonal antibody, clone M135
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of STAT1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y701 of human STAT1.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This peptide sequence has high homology to the conserved tyrosine site in rat and mouse stat1.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of human A-431 cells untreated (lane 1) or treated with EGF (100 nM) for 60 min (lane 2). The blots were probed with STAT1 (phospho Y701) monoclonal antibody, clone M135 (Cat # MAB1374).Enzyme-linked Immunoabsorbent Assay
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Gene Info — STAT1
Entrez GeneID
6772Gene Name
STAT1
Gene Alias
DKFZp686B04100, ISGF-3, STAT91
Gene Description
signal transducer and activator of transcription 1, 91kDa
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000165047|signal transducer and activator of transcription 1|signal transducer and activator of transcription-1|transcription factor ISGF-3 components p91/p84
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Interactome
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Pathway
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Disease
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Publication Reference
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STATs and gene regulation.
Darnell JE Jr.
Science 1997 Sep; 277(5332):1630.
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Transcription factor p91 interacts with the epidermal growth factor receptor and mediates activation of the c-fos gene promoter.
Fu XY, Zhang JJ.
Cell 1993 Sep; 74(6):1135.
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STATs and gene regulation.
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