ITGB4 monoclonal antibody, clone M126
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of A-431 cells serum starved overnight (lane 1) and treated with pervanadate (1mM) for 30 min (lane 2). The blots were probed with ITGB4 monoclonal antibody, clone M126 (Cat # MAB1371).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ITGB4.
Immunogen
Recombinant protein corresponding to cytoplasmic region of human ITGB4.
Host
Mouse
Reactivity
Human
Specificity
This sequence is found in all three integrin-beta 4 isoforms and has 90% homology with rat and mouse integrin-beta 4.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:2000)
Immunocytochemistry (1:250)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of A-431 cells serum starved overnight (lane 1) and treated with pervanadate (1mM) for 30 min (lane 2). The blots were probed with ITGB4 monoclonal antibody, clone M126 (Cat # MAB1371).Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — ITGB4
Entrez GeneID
3691Protein Accession#
NP_001005731Gene Name
ITGB4
Gene Alias
CD104
Gene Description
integrin, beta 4
Gene Ontology
HyperlinkGene Summary
Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD104 antigen|GP150|integrin beta 4|integrin beta-4 subunit
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Interactome
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Pathway
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Disease
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Publication Reference
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Identification of insulin receptor substrate 1 (IRS-1) and IRS-2 as signaling intermediates in the alpha6beta4 integrin-dependent activation of phosphoinositide 3-OH kinase and promotion of invasion.
Shaw LM.
Molecular and Cellular Biology 2001 Aug; 21(15):5082.
Application：Flow Cyt, Func, WB-Tr, Human, MDA-MB-435, T-47D cells.
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Tyrosine phosphorylation of the beta 4 integrin cytoplasmic domain mediates Shc signaling to extracellular signal-regulated kinase and antagonizes formation of hemidesmosomes.
Dans M, Gagnoux-Palacios L, Blaikie P, Klein S, Mariotti A, Giancotti FG.
J Biol Chem 2001 Jan; 276(2):1494.
Application：IF, WB-Tr, Human, Rat, HUVECs, 804G cells.
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Identification of insulin receptor substrate 1 (IRS-1) and IRS-2 as signaling intermediates in the alpha6beta4 integrin-dependent activation of phosphoinositide 3-OH kinase and promotion of invasion.
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