PTK2 (phospho Y397) monoclonal antibody, clone M121
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HUVECs untreated (lane 1) or treated with alkaline phosphatase (lane 2). Blots were probed with PTK2 (phospho Y397) monoclonal antibody, clone M121 (Cat # MAB1370).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of PTK2.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y397 of human PTK2.
Host
Mouse
Reactivity
Human, Mouse, Rabbit, Rat
Specificity
This peptide sequence has high homology to the conserved tyrosine site in rat and mouse FAK.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HUVECs untreated (lane 1) or treated with alkaline phosphatase (lane 2). Blots were probed with PTK2 (phospho Y397) monoclonal antibody, clone M121 (Cat # MAB1370).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PTK2
Entrez GeneID
5747Gene Name
PTK2
Gene Alias
FADK, FAK, FAK1, pp125FAK
Gene Description
PTK2 protein tyrosine kinase 2
Omim ID
600758Gene Ontology
HyperlinkGene Summary
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. [provided by RefSeq
Other Designations
focal adhesion kinase 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Focal adhesion kinase: a regulator of focal adhesion dynamics and cell movement.
Parsons JT, Martin KH, Slack JK, Taylor JM, Weed SA.
Oncogene 2000 Nov; 19(49):5606.
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Integrin-mediated signal transduction linked to Ras pathway by GRB2 binding to focal adhesion kinase.
Schlaepfer DD, Hanks SK, Hunter T, van der Geer P.
Nature 1994 Dec; 372(6508):786.
Application：WB-Ce, Mouse, NIH/3T3 cells.
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Autophosphorylation of the focal adhesion kinase, pp125FAK, directs SH2-dependent binding of pp60src.
Schaller MD, Hildebrand JD, Shannon JD, Fox JW, Vines RR, Parsons JT.
Molecular and Cellular Biology 1994 Mar; 14(3):1680.
Application：IP, WB-Tr, Chicken, CE cells.
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Focal adhesion kinase: a regulator of focal adhesion dynamics and cell movement.
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