PRKCG monoclonal antibody, clone M110
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of PRKCG isoforms in neonatal rat brain lysate. The rat brain blot was probed with PRKCG monoclonal antibody, clone M110 (Cat # MAB1367) at decreasing dilutions : Lane 1 = 1:250. Lane 2 = 1:500. Lane 3 = 1:1000.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant PRKCG.
Immunogen
Recombinant protein corresponding to amino acids 499-697 of human PRKCG.
Host
Mouse
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:2000)
Immunocytochemistry (1:100)
Immunoprecipitation (1-5 ul)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of PRKCG isoforms in neonatal rat brain lysate. The rat brain blot was probed with PRKCG monoclonal antibody, clone M110 (Cat # MAB1367) at decreasing dilutions : Lane 1 = 1:250. Lane 2 = 1:500. Lane 3 = 1:1000.Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKCG
Entrez GeneID
5582Gene Name
PRKCG
Gene Alias
MGC57564, PKC-gamma, PKCC, PKCG, SCA14
Gene Description
protein kinase C, gamma
Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Publication Reference
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A Ras activation pathway dependent on Syk phosphorylation of protein kinase C.
Kawakami Y, Kitaura J, Yao L, McHenry RW, Kawakami Y, Newton AC, Kang S, Kato RM, Leitges M, Rawlings DJ, Kawakami T.
PNAS 2003 Aug; 100(16):9470.
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The molecular heterogeneity of protein kinase C and its implications for cellular regulation.
Nishizuka Y.
Nature 1988 Aug; 334(6184):661.
Application：IF, IHC, Rat, Rat cerebellar cortex.
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A Ras activation pathway dependent on Syk phosphorylation of protein kinase C.
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