PXN (phospho Y31) monoclonal antibody, clone M102
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of A-431 cells (30 ug/lane) serum starved overnight (lane 1) or treated with pervanadate (1 mmL) for 30 min (lane 2). The blot was probed with PXN (phospho Y31) monoclonal antibody, clone M102 (Cat # MAB1365).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of PXN.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y31 of human PXN.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This human sequence is highly conserved in rat and mouse paxillin.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of A-431 cells (30 ug/lane) serum starved overnight (lane 1) or treated with pervanadate (1 mmL) for 30 min (lane 2). The blot was probed with PXN (phospho Y31) monoclonal antibody, clone M102 (Cat # MAB1365).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PXN
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Interactome
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Pathway
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Disease
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Publication Reference
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Multiple stimuli induce tyrosine phosphorylation of the Crk-binding sites of paxillin.
Schaller MD, Schaefer EM.
The Biochemical Journal 2001 Nov; 360(Pt 1):57.
Application：WB-Ce, WB-Tr, Chicken, Rat, Chicken embryo (CE) cells, GN4 cells.
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CRKL links p210BCR/ABL with paxillin in chronic myelogenous leukemia cells.
Salgia R, Uemura N, Okuda K, Li JL, Pisick E, Sattler M, de Jong R, Druker B, Heisterkamp N, Chen LB, et al..
The Journal of Biological Chemistry 1995 Dec; 270(49):29145.
Application：WB-Re, Recombinant protein.
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Multiple stimuli induce tyrosine phosphorylation of the Crk-binding sites of paxillin.
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