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Last updated: 2016/11/27

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CA9 monoclonal antibody, clone CA9/781

  • Catalog # : MAB13458
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant human CA9.
  • Immunogen:
  • Recombinant protein corresponding to full length human CA9.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 55
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A/G purification
  • Isotype:
  • IgG2b, kappa
  • Recommend Usage:
  • Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
    Immunofluorescence (1-2 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
    Western Blot (0.5-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM PBS.
  • Storage Instruction:
  • Store at -20 to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of HCT-116 cell lysate with CA9 monoclonal antibody, clone CA9/781 (Cat # MAB13458).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human renal cell carcinoma with CA9 monoclonal antibody, clone CA9/781 (Cat # MAB13458).
  • Immunofluorescence
  • Flow Cytometry
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunofluorescence
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 768
  • Gene Name:
  • CA9
  • Gene Alias:
  • CAIX,MN
  • Gene Description:
  • carbonic anhydrase IX
  • Gene Summary:
  • Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000022773,RCC-associated protein G250,carbonic dehydratase
  • Gene Pathway
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