VIM monoclonal antibody, clone LN-6
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human melanoma with VIM monoclonal antibody, clone LN-6 (Cat # MAB13451).
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Specification
Product Description
Mouse monoclonal antibody raised against native human VIM.
Immunogen
Human thymic nuclear extract.
Host
Mouse
Theoretical MW (kDa)
57-60
Reactivity
Human
Specificity
This monoclonal antibody reacts with a non-hematopoietic epitope of VIM and shows no cross-reaction with other closely related intermediate filament proteins such as desmin, keratin, neurofilament, and glial fibrillary acid protein.
Form
Liquid
Purification
PEG precipitation
Isotype
IgM
Recommend Usage
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
Immunofluorescence (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS (0.05% BSA, 0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human melanoma with VIM monoclonal antibody, clone LN-6 (Cat # MAB13451).Immunofluorescence
Flow Cytometry
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Gene Info — VIM
Entrez GeneID
7431Protein Accession#
P08670Gene Name
VIM
Gene Alias
FLJ36605
Gene Description
vimentin
Omim ID
193060Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract
Other Designations
OTTHUMP00000019224
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Interactome
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Disease
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