TGFA monoclonal antibody, clone 1E8-G6
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Specifications
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human TGFA.
Immunogen
A synthetic peptide corresponding to amino acids 34-43 of human TGFA.
Sequence
PPVAAAVVSH
Host
Mouse
Theoretical MW (kDa)
~6
Reactivity
Human
Specificity
This monoclonal antibody reacts with the TGFA and shows no cross-reaction with EGF and the neuropeptide synenkephalin.
Form
Liquid
Isotype
IgM, kappa
Recommend Usage
Flow Cytometry (1-2 ug/million cells)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testicle tumor with TGFA monoclonal antibody, clone 1E8-G6 (Cat # MAB13444).Immunofluorescence
Flow Cytometry
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Gene Info — TGFA
Entrez GeneID
7039Protein Accession#
P01135Gene Name
TGFA
Gene Alias
TFGA
Gene Description
transforming growth factor, alpha
Omim ID
190170Gene Ontology
HyperlinkGene Summary
Transforming growth factors (TGFs) are biologically active polypeptides that reversibly confer the transformed phenotype on cultured cells. TGF-alpha shows about 40% sequence homology with epidermal growth factor (EGF; MIM 131530) and competes with EGF for binding to the EGF receptor (MIM 131550), stimulating its phosphorylation and producing a mitogenic response.[supplied by OMIM
Other Designations
transforming growth factor-alpha
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Interactomes
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Pathways
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Diseases
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