NCL monoclonal antibody, clone 364-5
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Specification
Product Description
Mouse monoclonal antibody raised against native human NCL.
Immunogen
DHL-1 nuclear extract.
Host
Mouse
Theoretical MW (kDa)
76
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
Immunofluorescence (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL)
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS.
Storage Instruction
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skin (A), human tonsil (B) with NCL monoclonal antibody, clone 364-5 (Cat # MAB13384) and human colon carcinoma (C) with AF488 conjugated format of NCL monoclonal antibody, clone 364-5 (Cat # MAB13384).Immunofluorescence
Flow Cytometry
Flow cytometric analysis of HEK 293T cells with NCL monoclonal antibody, clone 364-5 (Cat # MAB13384). Black: Cells alone; Grey: Isotype control; Green: AF488 conjugated format of NCL monoclonal antibody, clone 364-5. -
Gene Info — NCL
Entrez GeneID
4691Protein Accession#
P19338Gene Name
NCL
Gene Alias
C23, FLJ45706
Gene Description
nucleolin
Omim ID
164035Gene Ontology
HyperlinkGene Summary
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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