Mouse monoclonal antibody raised against native human NCL.
DHL-1 nuclear extract.
Theoretical MW (kDa):
Protein A/G purification
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL) Immunofluorescence (0.5-1 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL) Western Blot (0.5-1 ug/mL) The optimal working dilution should be determined by the end user.
In 10 mM PBS.
Store at -20 to -80°C. Aliquot to avoid repeated freezing and thawing.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skin (A), human tonsil (B) with NCL monoclonal antibody, clone 364-5 (Cat # MAB13384) and human colon carcinoma (C) with AF488 conjugated format of NCL monoclonal antibody, clone 364-5 (Cat # MAB13384).
Flow cytometric analysis of HEK 293T cells with NCL monoclonal antibody, clone 364-5 (Cat # MAB13384). Black: Cells alone; Grey: Isotype control; Green: AF488 conjugated format of NCL monoclonal antibody, clone 364-5.
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq