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CD99 monoclonal antibody, clone HO36-1.1

  • Catalog # : MAB13349
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against native human CD99.
  • Immunogen:
  • Purified E-rosette forming cells from human peripheral blood lymphocytes.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 27-32
  • Reactivity:
  • Human
  • Specificity:
  • This monoclonal antibody shows a very similar reactivity to other CD99 monoclonal antibodies.
  • Form:
  • Liquid
  • Purification:
  • Protein A/G purification
  • Isotype:
  • IgM, kappa
  • Recommend Usage:
  • Flow Cytometry (5-10 uL/106 cells in 0.1 mL)
    Immunofluorescence (0.5-1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM PBS (0.05% BSA, 0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human Ewing's sarcoma (A) and human testicular carcinoma (B) with CD99 monoclonal antibody, clone HO36-1.1 (Cat # MAB13349).
  • Immunofluorescence
  • Flow Cytometry
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunofluorescence
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 4267
  • Gene Name:
  • CD99
  • Gene Alias:
  • Gene Description:
  • CD99 molecule
  • Gene Summary:
  • The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • CD99 antigen,E2 antigen,MIC2 (monoclonal antibody 12E7),OTTHUMP00000022840,T-cell surface glycoprotein E2,antigen identified by monoclonal 12E7, Y homolog,antigen identified by monoclonal antibodies 12E7, F21 and O13,surface antigen MIC2
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