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EPCAM monoclonal antibody, clone EGP40/837

  • Catalog # : MAB13335
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant human EPCAM.
  • Immunogen:
  • Recombinant protein corresponding to full length human EPCAM.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 40-43
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A/G purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
    Immunofluorescence (1-2 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
    Western Blot (0.5-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM PBS (0.05% BSA, 0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of HCT-116 cell lysate with EPCAM monoclonal antibody, clone EGP40/837 (Cat # MAB13335).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon carcinoma (A), human ovarian carcinoma (B) and human endometrial carcinoma (C) with EPCAM monoclonal antibody, clone EGP40/837 (Cat # MAB13335).
  • Immunofluorescence
  • Immunofluorescence
  • Confocal Immunofluorescent staining of SK-OV-3 cells with AF488 conjugated format of EPCAM monoclonal antibody, clone EGP40/837 (Cat # MAB13335) (Green) (A) and AF488 conjugated format of Isotype Control Monoclonal Antibody (IgG1) (Green) (B). DAPI was used to stain the cell nuclei (Blue).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of MCF-7 cells with EPCAM monoclonal antibody, clone EGP40/837 (Cat # MAB13335). Black: Cells alone; Green: Isotype control; Red: PE conjugated format of EPCAM monoclonal antibody, clone EGP40/837.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4072
  • Gene Name:
  • EPCAM
  • Gene Alias:
  • 17-1A,323/A3,CD326,CO-17A,CO17-1A,EGP,EGP-2,EGP34,EGP40,ESA,Ep-CAM,GA733-2,HEA125,KS1/4,KSA,M4S1,MH99,MIC18,MK-1,MOC31,TACST-1,TACSTD1,TROP1,hEGP-2
  • Gene Description:
  • epithelial cell adhesion molecule
  • Gene Summary:
  • This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq
  • Other Designations:
  • adenocarcinoma-associated antigen,carcinoma-associated antigen GA733-2,human epithelial glycoprotein-2,membrane component, chromosome 4, surface marker (35kD glycoprotein),tumor-associated calcium signal transducer 1
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