Mouse monoclonal antibody raised against synthetic peptide of human EPCAM.
A synthetic peptide corresponding to amino acids 20-60 at N-terminus region of human EPCAM.
Theoretical MW (kDa):
Protein A/G purification
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL) Immunofluorescence (1-2 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL) Western Blot (0.5-1 ug/mL) The optimal working dilution should be determined by the end user.
In 10 mM PBS.
Store at -20 to -80°C. Aliquot to avoid repeated freezing and thawing.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with EPCAM monoclonal antibody, clone EGP40/826 (Cat # MAB13334).
Confocal Immunofluorescent staining of SK-OV-3 cells with AF488 conjugated format of EPCAM monoclonal antibody, clone EGP40/826 (Cat # MAB13334) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (Red). DAPI was used to stain the cell nuclei (Blue) (A). Confocal Immunofluorescent staining of SK-OV-3 cells with AF488 conjugated format of Isotype Control Monoclonal Antibody (IgG1) (Green). DAPI was used to stain the cell nuclei (Blue) (B).
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq