Blood Group Antigen A monoclonal antibody, clone 3-3A
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer with Blood Group Antigen A monoclonal antibody, clone 3-3A (Cat # MAB13298).
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Specification
Product Description
Mouse monoclonal antibody raised against native human Blood Group Antigen A.
Immunogen
Mucin isolated from an ovarian cyst fluid.
Host
Mouse
Reactivity
Human
Specificity
This monoclonal antibody preferably reacts with determinants of chain A and H type 3 (Gal1-3GalNAc-R) and 4 (Gal1-3GalNAc-R), but not with type 1 and 2 chain structures. It is not reactive with immuno-dominant A trisaccharide. This antibody is applicable for tissue staining in tumor patients with blood groups A and AB. It shows a highly heterogeneous reactivity in human colon tumor tissue and adjacent mucosa.
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
Immunofluorescence (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS.
Storage Instruction
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer with Blood Group Antigen A monoclonal antibody, clone 3-3A (Cat # MAB13298).Immunofluorescence
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