Blood Group Antigen A monoclonal antibody, clone HE-193
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Specification
Product Description
Mouse monoclonal antibody raised against native human Blood Group Antigen A.
Immunogen
A mixture of erythrocytes of Blood Group Antigen A and glycoprotein fraction isolated from the saliva of secretors with Blood Group Antigen A.
Host
Mouse
Reactivity
Human
Specificity
This monoclonal antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssmann antigen.
Form
Liquid
Isotype
IgM, kappa
Recommend Usage
Agglutination
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS (0.05% sodium azide).
Storage Instruction
Store at -20°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer with Blood Group Antigen A monoclonal antibody, clone HE-193 (Cat # MAB13294).Immunofluorescence
Agglutination
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