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FBXO5 monoclonal antibody, clone EMI1/1176

  • Catalog # : MAB13276
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human FBXO5.
  • Immunogen:
  • Recombinant protein corresponding to 203 residues around amino acids 1-250 of human FBXO5.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 56
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A/G purification
  • Isotype:
  • IgG2a, kappa
  • Recommend Usage:
  • Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
    Immunofluorescence (0.5-1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
    Western Blot (1-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 1 mM PBS.
  • Storage Instruction:
  • Store at -20 to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: HeLa, Lane 2: HepG2, Lane 3: HEK293 and Lane 4: K562 cell lysates with FBXO5 monoclonal antibody, clone EMI1/1176 (Cat # MAB13276).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human ovarian cancer (A, B) with FBXO5 monoclonal antibody, clone EMI1/1176 (Cat # MAB13276).
  • Immunofluorescence
  • Flow Cytometry
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunofluorescence
  • Flow Cytometry
  • Gene Information
  • Gene Name:
  • FBXO5
  • Gene Alias:
  • EMI1,FBX5,Fbxo31
  • Gene Description:
  • F-box protein 5
  • Gene Summary:
  • This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
  • Other Designations:
  • F-box only protein 5,F-box protein Fbx5,OTTHUMP00000017453,early mitotic inhibitor 1
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