FBXO5 monoclonal antibody, clone EMI1/1176
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: HeLa, Lane 2: HepG2, Lane 3: HEK293 and Lane 4: K562 cell lysates with FBXO5 monoclonal antibody, clone EMI1/1176 (Cat # MAB13275).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human ovarian cancer (A, B) with FBXO5 monoclonal antibody, clone EMI1/1176 (Cat # MAB13275).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human FBXO5.
Immunogen
Recombinant protein corresponding to 203 residues around amino acids 1-250 of human FBXO5.
Host
Mouse
Theoretical MW (kDa)
56
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
Immunofluorescence (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 1 mM PBS (0.05% BSA, 0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: HeLa, Lane 2: HepG2, Lane 3: HEK293 and Lane 4: K562 cell lysates with FBXO5 monoclonal antibody, clone EMI1/1176 (Cat # MAB13275).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human ovarian cancer (A, B) with FBXO5 monoclonal antibody, clone EMI1/1176 (Cat # MAB13275).Immunofluorescence
Flow Cytometry
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Gene Info — FBXO5
Entrez GeneID
26271Protein Accession#
Q9UKT4Gene Name
FBXO5
Gene Alias
EMI1, FBX5, Fbxo31
Gene Description
F-box protein 5
Omim ID
606013Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
F-box only protein 5|F-box protein Fbx5|OTTHUMP00000017453|early mitotic inhibitor 1
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