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CDKN1B monoclonal antibody, clone KIP1/769

  • Catalog # : MAB13246
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant human CDKN1B.
  • Immunogen:
  • Recombinant protein corresponding to full length human CDKN1B.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 25-26
  • Reactivity:
  • Human, Rat
  • Specificity:
  • This monoclonal antibody is highly specific to CDKN1B and shows no cross-reaction with other closely related mitotic inhibitors.
  • Form:
  • Liquid
  • Purification:
  • Protein A/G purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
    Immunofluorescence (0.5-1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL)
    Western Blot (0.5-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM PBS.
  • Storage Instruction:
  • Store at -20 to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon carcinoma (A), human prostate cancer (B), human cervical cancer (C) and rat colon (D) with CDKN1B monoclonal antibody, clone KIP1/769 (Cat # MAB13246).
  • Immunofluorescence
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of Jurkat cells with CDKN1B monoclonal antibody, clone KIP1/769 (Cat # MAB13246). Black: Cells alone; Grey: Isotype control; Green: AF488 conjugated format of CDKN1B monoclonal antibody, clone KIP1/769.
  • Application Image
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunofluorescence
  • Gene Information
  • Entrez GeneID:
  • 1027
  • Gene Name:
  • CDKN1B
  • Gene Alias:
  • CDKN4,KIP1,MEN1B,MEN4,P27KIP1
  • Gene Description:
  • cyclin-dependent kinase inhibitor 1B (p27, Kip1)
  • Gene Summary:
  • This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq
  • Other Designations:
  • cyclin-dependent kinase inhibitor 1B
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