GPC3 monoclonal antibody, clone SPM595
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human GPC3.
Immunogen
Recombinant protein corresponding to amino acids 511-580 of human GPC3.
Host
Mouse
Theoretical MW (kDa)
67
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/million cells in 0.1 mL)
Immunofluorescence (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 1 mM PBS (0.05% BSA, 0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human hepatocellular carcinoma (A) and human melanoma (B) using GPC3 monoclonal antibody, clone SPM595 (Cat # MAB13056).Immunofluorescence
Flow Cytometry
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Gene Info — GPC3
Entrez GeneID
2719Protein Accession#
P51654Gene Name
GPC3
Gene Alias
DGSX, OCI-5, SDYS, SGB, SGBS, SGBS1
Gene Description
glypican 3
Gene Ontology
HyperlinkGene Summary
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000024058|OTTHUMP00000062492|glypican proteoglycan 3
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