MAPRE2 monoclonal antibody, clone 1F3
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of HEK293T cells transfected with the Lane 1: pCMV6-ENTRY control and Lane 2: pCMV6-ENTRY MAPRE2 cDNA for 48 hrs (5 ug/lane) using MAPRE2 monoclonal antibody, clone 1F3 (Cat # MAB12998).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex with MAPRE2 monoclonal antibody, clone 1F3 (Cat # MAB12998) at 20 ug/mL working concentration.
Immunofluorescence
Immunofluorescent staining of COS-7 cells with MAPRE2 monoclonal antibody, clone 1F3 (Cat # MAB12998) transiently transfected by pCMV6-ENTRY MAPRE2.
Flow Cytometry
Flow cytometric analysis of HEK 293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) using MAPRE2 monoclonal antibody, clone 1F3 (Cat # MAB12998) (A); Jurkat cells (B) and HeLa cells (C) transfected using MAPRE2 monoclonal antibody, clone 1F3 (Cat # MAB12998) (Red) compared to a nonspecific negative control antibody (Blue).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human MAPRE2.
Immunogen
Recombinant protein corresponding to full length human MAPRE2.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG2a
Recommend Usage
Flow Cytometry (1:100)
Immunofluorescence (1:100)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (20 ug/mL)
Western Blot (1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.3 (50% glycerol, 1% BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of HEK293T cells transfected with the Lane 1: pCMV6-ENTRY control and Lane 2: pCMV6-ENTRY MAPRE2 cDNA for 48 hrs (5 ug/lane) using MAPRE2 monoclonal antibody, clone 1F3 (Cat # MAB12998).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex with MAPRE2 monoclonal antibody, clone 1F3 (Cat # MAB12998) at 20 ug/mL working concentration.Immunofluorescence
Immunofluorescent staining of COS-7 cells with MAPRE2 monoclonal antibody, clone 1F3 (Cat # MAB12998) transiently transfected by pCMV6-ENTRY MAPRE2.Flow Cytometry
Flow cytometric analysis of HEK 293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) using MAPRE2 monoclonal antibody, clone 1F3 (Cat # MAB12998) (A); Jurkat cells (B) and HeLa cells (C) transfected using MAPRE2 monoclonal antibody, clone 1F3 (Cat # MAB12998) (Red) compared to a nonspecific negative control antibody (Blue). -
Gene Info — MAPRE2
Entrez GeneID
10982Protein Accession#
Q15555Gene Name
MAPRE2
Gene Alias
EB1, EB2, RP1
Gene Description
microtubule-associated protein, RP/EB family, member 2
Omim ID
605789Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. The function of this protein is unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. This gene may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
APC-binding protein EB1|T-cell activation protein, EB1 family
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Interactome
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Disease
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