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DCX monoclonal antibody, clone 2G5

  • Catalog # : MAB12993
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human DCX.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 362-411 of human DCX.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Flow Cytometry (1:200-1:400)
    Immunocytochemistry (1:200-1:1000)
    Immunofluorescence (1:1000)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% protein stabilizer, 0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western Blot analysis of DCX recombinant protein with DCX monoclonal antibody, clone 2G5 (Cat # MAB12993).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex with DCX monoclonal antibody, clone 2G5 (Cat # MAB12993).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of HepG2 cells with DCX monoclonal antibody, clone 2G5 (Cat # MAB12993) (Green). Blue: DRAQ5 fluorescent DNA dye.
  • ELISA
  • Flow Cytometry
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • ELISA
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 1641
  • Gene Name:
  • DCX
  • Gene Alias:
  • DBCN,DC,LISX,SCLH,XLIS
  • Gene Description:
  • doublecortin
  • Gene Summary:
  • In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The protein encoded by this gene is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. The encoded protein contains two doublecortin domains, which bind microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene are a cause of X-linked lissencephaly. Multiple transcript variants encoding at least three different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000062892,doublecortex,doublin,lissencephalin-X
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