ACTG1 monoclonal antibody, clone 2A3
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human ACTG1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to acetylated N-terminus region of human ACTG1.
Sequence
Ac-EEEIAALVI-COOH
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunohistochemistry (Frozen sections) (1:100-1:500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 ug/mL)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human small intestine with ACTG1 monoclonal antibody, clone 2A3 (Cat # MAB12987) at 5 ug/mL working concentration.Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemistry staining of methanol fixed human dermal fibroblasts with ACTG1 monoclonal antibody, clone 2A3 (Cat # MAB12987) at 1:500 dilution.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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Gene Info — ACTG1
Entrez GeneID
71Protein Accession#
P63261Gene Name
ACTG1
Gene Alias
ACT, ACTG, DFNA20, DFNA26
Gene Description
actin, gamma 1
Gene Ontology
HyperlinkGene Summary
Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells. [provided by RefSeq
Other Designations
actin, cytoplasmic 2|actin, gamma 1 propeptide|cytoskeletal gamma-actin
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Interactome
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Pathway
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