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Last updated: 2016/12/4

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ACTB monoclonal antibody, clone 4C2

  • Catalog # : MAB12986
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of human ACTB.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to acetylated N-terminus region of human ACTB.
  • Sequence:
  • Ac-DDDIAALVV-COOH
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA
    Flow Cytometry
    Immunocytochemistry
    Immunohistochemistry (Frozen sections) (1:100-1:500)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 ug/mL)
    Western Blot (1:1000-1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide).
  • Storage Instruction:
  • Store at 4°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human prostate with ACTB monoclonal antibody, clone 4C2 (Cat # MAB12986) at 5 ug/mL working concentration.
  • Immunohistochemistry (Frozen sections)
  • Immunohistochemistry (Frozen sections)
  • Immunohistochemical staining (Frozen sections) of 3 days (A) and 1 month (B) old zebrafish embryo with ACTB monoclonal antibody, clone 4C2 (Cat # MAB12986) visualized by IF detection system.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemistry staining of methanol fixed human dermal fibroblasts with ACTB monoclonal antibody, clone 4C2 (Cat # MAB12986) at 1:500 dilution.
  • ELISA
  • Flow Cytometry
  • Application Image
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry (Frozen sections)
  • Immunohistochemistry (Frozen sections)
  • enlarge
  • ELISA
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 60
  • Gene Name:
  • ACTB
  • Gene Alias:
  • PS1TP5BP1
  • Gene Description:
  • actin, beta
  • Gene Summary:
  • This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
  • Other Designations:
  • PS1TP5-binding protein 1,actin, cytoplasmic 1,beta actin,beta cytoskeletal actin
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