VCP monoclonal antibody, clone 4G9
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: HeLa cells, Lane 2: Jurkat cells, Lane 3: MCF7 cells and Lane 4: mouse splenocytes with VCP monoclonal antibody, clone 4G9 (Cat # MAB12952).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human prostate with VCP monoclonal antibody, clone 4G9 (Cat # MAB12952) at 5 ug/mL working concentration.
Immunocytochemistry
Immunocytochemistry staining of nocodazole treated Hela cells overnight with VCP monoclonal antibody, clone 4G9 (Cat # MAB12952) followed by Cy3-conjugated goat anti-mouse IgG and DAPI.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human VCP.
Immunogen
Recombinant protein corresponding to human VCP.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG1
Recommend Usage
Immunocytochemistry
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 ug/mL)
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.09% sodium azide).
Storage Instruction
Store at 4°C. Do not Freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: HeLa cells, Lane 2: Jurkat cells, Lane 3: MCF7 cells and Lane 4: mouse splenocytes with VCP monoclonal antibody, clone 4G9 (Cat # MAB12952).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human prostate with VCP monoclonal antibody, clone 4G9 (Cat # MAB12952) at 5 ug/mL working concentration.Immunocytochemistry
Immunocytochemistry staining of nocodazole treated Hela cells overnight with VCP monoclonal antibody, clone 4G9 (Cat # MAB12952) followed by Cy3-conjugated goat anti-mouse IgG and DAPI.Immunofluorescence
Immunoprecipitation
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Gene Info — VCP
Entrez GeneID
7415Protein Accession#
P55072Gene Name
VCP
Gene Alias
IBMPFD, MGC131997, MGC148092, MGC8560, TERA, p97
Gene Description
valosin-containing protein
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq
Other Designations
transitional endoplasmic reticulum ATPase|yeast Cdc48p homolog
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Interactome
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Disease
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