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VCP monoclonal antibody, clone 4G9

  • Catalog # : MAB12952
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant human VCP.
  • Immunogen:
  • Recombinant protein corresponding to human VCP.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Immunocytochemistry
    Immunofluorescence (1-4 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 ug/mL)
    Immunoprecipitation
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (0.09% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: HeLa cells, Lane 2: Jurkat cells, Lane 3: MCF7 cells and Lane 4: mouse splenocytes with VCP monoclonal antibody, clone 4G9 (Cat # MAB12952).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human prostate with VCP monoclonal antibody, clone 4G9 (Cat # MAB12952) at 5 ug/mL working concentration.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemistry staining of nocodazole treated Hela cells overnight with VCP monoclonal antibody, clone 4G9 (Cat # MAB12952) followed by Cy3-conjugated goat anti-mouse IgG and DAPI.
  • Immunofluorescence
  • Immunoprecipitation
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunofluorescence
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 7415
  • Gene Name:
  • VCP
  • Gene Alias:
  • IBMPFD,MGC131997,MGC148092,MGC8560,TERA,p97
  • Gene Description:
  • valosin-containing protein
  • Gene Summary:
  • The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq
  • Other Designations:
  • transitional endoplasmic reticulum ATPase,yeast Cdc48p homolog
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