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PARP1 monoclonal antibody, clone 5F1

  • Catalog # : MAB12896
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant human PARP1.
  • Immunogen:
  • Recombinant protein corresponding to full length human PARP1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Immunoaffinity purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
    Western Blot (1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.3 (50% glycerol, 1% BSA, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of HEK293T cells transfected with the Lane 1: pCMV6-ENTRY control and Lane 2: pCMV6-ENTRY PARP1 cDNA for 48 hrs (5 ug/lane) using PARP1 monoclonal antibody, clone 5F1 (Cat # MAB12896).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with PARP1 monoclonal antibody, clone 5F1 (Cat # MAB12896).
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 142
  • Gene Name:
  • PARP1
  • Gene Alias:
  • ADPRT,ADPRT1,PARP,PARP-1,PPOL,pADPRT-1
  • Gene Description:
  • poly (ADP-ribose) polymerase 1
  • Gene Summary:
  • This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq
  • Other Designations:
  • ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase),ADP-ribosyltransferase NAD(+),OTTHUMP00000035663,poly (ADP-ribose) polymerase family, member 1,poly(ADP-ribose) polymerase,poly(ADP-ribose) synthetase,poly(ADP-ribosyl)transferase
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