Western Blot analysis of HEK293T cells transfected with the Lane 1: pCMV6-ENTRY control and Lane 2: pCMV6-ENTRY PARP1 cDNA for 48 hrs (5 ug/lane) using PARP1 monoclonal antibody, clone 5F1 (Cat # MAB12896).
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq