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Last updated: 2017/6/25

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Tsc2 monoclonal antibody, clone 249CT19.1.3

  • Catalog # : MAB12866
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant mouse Tsc2.
  • Immunogen:
  • Recombinant protein corresponding to mouse Tsc2.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Immunofluorescence (1:10-1:50)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50)
    Western Blot (1:100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of mouse NIH/3T3 cell line lysate (35 ug/lane) with Tsc2 monoclonal antibody, clone 249CT19.1.3 (Cat # MAB12866).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human liver with Tsc2 monoclonal antibody, clone 249CT19.1.3 (Cat # MAB12866) at 1:50 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of HeLa cells with Tsc2 monoclonal antibody, clone 249CT19.1.3 (Cat # MAB12866) at 1:25 dilution followed by Alexa Fluor 488-conjugated donkey anti-mouse secondary antibody (Green) at 1:400 dilution. Cytoplasmic actin was counterstained with Alexa Fluor 555-conjugated Phalloidin (Red) at 7 units/mL working concentration 1 hr. Nuclei were counterstained with DAPI (Blue) at 10 ug/mL working concentration 10 min.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Gene Name:
  • Tsc2
  • Gene Alias:
  • Tcs2
  • Gene Description:
  • tuberous sclerosis 2
  • Other Designations:
  • tuberin
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