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ARHGAP25 monoclonal antibody, clone 12G4

  • Catalog # : MAB12855
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant human ARHGAP25.
  • Immunogen:
  • Recombinant protein corresponding to full length human ARHGAP25.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A/G purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Flow Cytometry (1:100)
    Immunofluorescence (1:100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.3 (50% glycerol, 1% BSA, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: HepG2, Lane 2: HeLa, Lane 3: HT29, Lane 4: A549, Lane 5: COS7, Lane 6: Jurkat, Lane 7: MDCK, Lane 8: PC12 and Lane 9: MCF7 (35 ug/lane) with ARHGAP25 monoclonal antibody, clone 12G4 (Cat # MAB12855).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human spleen with ARHGAP25 monoclonal antibody, clone 12G4 (Cat # MAB12855) at 10 ug/mL working concentration.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of COS-7 cells with ARHGAP25 monoclonal antibody, clone 12G4 (Cat # MAB12855) transiently transfected by pCMV6-ENTRY ARHGAP25.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HEK 293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) using ARHGAP25 monoclonal antibody, clone 12G4 (Cat # MAB12855).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 9938
  • Gene Name:
  • ARHGAP25
  • Gene Alias:
  • KAIA0053
  • Gene Description:
  • Rho GTPase activating protein 25
  • Gene Summary:
  • ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM
  • Other Designations:
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