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Histone H3 (acetyl K18) monoclonal antibody, clone RM166

  • Catalog # : MAB12840
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic acetyl peptide of human histone H3 (acetyl K18).
  • Immunogen:
  • A synthetic acetyl peptide corresponding to residues surrounding K18 of human histone H3.
  • Host:
  • Rabbit
  • Specificity:
  • This antibody reacts to histone H3 acetylated at Lysine 18. No cross reactivity with other acetylated Lysines in histone H3.
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ChIP (2-10 ug/mL)
    ELISA (0.2-1 ug/mL)
    Immunocytochemistry (0.5-2 ug/mL)
    Immunohistochemistry (1-10 ug/mL)
    Western Blot (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • ChIP
  • ChIP analysis of HeLa cells with or without sodium butyrate treatment using Histone H3 (acetyl K18) monoclonal antibody, clone RM166 (Cat # MAB12840) under 5 ug working concentration. Real-time PCR was performed using primers specific to the gene indicated.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: HeLa cells, Lane 2: HeLa cells treated with sodium butyrate using Histone H3 (acetyl K18) monoclonal antibody, clone RM166 (Cat # MAB12840) under 0.5 ug/mL working concentration.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of HepG2 cells with Histone H3 (acetyl K18) monoclonal antibody, clone RM166 (Cat # MAB12840) under 10 ug/mL working concentration.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemistry staining of HeLa cells treated with sodium butyrate using Histone H3 (acetyl K18) monoclonal antibody, clone RM166 (Cat # MAB12840) (Red) under 2 ug/mL working concentration. Actin filaments were stained with fluorescein phalloidin (Green).
  • ELISA
  • ELISA
  • Sandwich ELISA analysis of HeLa cells treated or untreated with sodium butyrate using Histone H3 (acetyl K18) monoclonal antibody, clone RM166 (Cat # MAB12840) as the detection antibody and biotinylated format of Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12804) as the capture antibody.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 8350
  • Gene Name:
  • HIST1H3A
  • Gene Alias:
  • H3/A,H3FA
  • Gene Description:
  • histone cluster 1, H3a
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
  • Other Designations:
  • H3 histone family, member A,histone 1, H3a
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