Histone H3 (acetyl K9) monoclonal antibody, clone RM161
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More Files
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ChIP
ChIP analysis of HeLa cells with Histone H3 (acetyl K9) monoclonal antibody, clone RM161 (Cat # MAB12839) under 5 ug working concentration. Real-time PCR was performed using primers specific to the gene indicated.
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cells, Lane 2: HeLa cells treated with sodium butyrate using Histone H3 (acetyl K9) monoclonal antibody, clone RM161 (Cat # MAB12839) under 0.25 ug/mL working concentration.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of HepG2 cells with Histone H3 (acetyl K9) monoclonal antibody, clone RM161 (Cat # MAB12839) under 0.5 ug/mL working concentration.
Immunocytochemistry
Immunocytochemistry staining of HeLa cells treated with sodium butyrate using Histone H3 (acetyl K9) monoclonal antibody, clone RM161 (Cat # MAB12839) (Red) under 1 ug/mL working concentration. Actin filaments were stained with fluorescein phalloidin (Green).
Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA analysis of HeLa cells treated or untreated with sodium butyrate using Histone H3 (acetyl K9) monoclonal antibody, clone RM161 (Cat # MAB12839) as the detection antibody under 1 ug/mL working concentration. Using biotinylated format of Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12804) as the capture antibody under 1 ug/mL working concentration.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human histone H3 (acetyl K9).
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic acetyl peptide corresponding to residues surrounding K9 of human Histone H3.
Sequence
N/A
Specificity
This antibody reacts to histone H3 acetylated at Lysine 9. No cross reactivity with other acetylated Lysines in histone H3.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ChIP (2-10 ug/mL)
ELISA (0.2-1 ug/mL)
Immunocytochemistry (0.5-2 ug/mL)
Immunohistochemistry (0.1-1 ug/mL)
Western Blot (0.25-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
ChIP analysis of HeLa cells with Histone H3 (acetyl K9) monoclonal antibody, clone RM161 (Cat # MAB12839) under 5 ug working concentration. Real-time PCR was performed using primers specific to the gene indicated.Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cells, Lane 2: HeLa cells treated with sodium butyrate using Histone H3 (acetyl K9) monoclonal antibody, clone RM161 (Cat # MAB12839) under 0.25 ug/mL working concentration.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of HepG2 cells with Histone H3 (acetyl K9) monoclonal antibody, clone RM161 (Cat # MAB12839) under 0.5 ug/mL working concentration.Immunocytochemistry
Immunocytochemistry staining of HeLa cells treated with sodium butyrate using Histone H3 (acetyl K9) monoclonal antibody, clone RM161 (Cat # MAB12839) (Red) under 1 ug/mL working concentration. Actin filaments were stained with fluorescein phalloidin (Green).Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA analysis of HeLa cells treated or untreated with sodium butyrate using Histone H3 (acetyl K9) monoclonal antibody, clone RM161 (Cat # MAB12839) as the detection antibody under 1 ug/mL working concentration. Using biotinylated format of Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12804) as the capture antibody under 1 ug/mL working concentration. -
Gene Info — HIST1H3A
Entrez GeneID
8350Gene Name
HIST1H3A
Gene Alias
H3/A, H3FA
Gene Description
histone cluster 1, H3a
Omim ID
602810Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H3 histone family, member A|histone 1, H3a
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