Rabbit monoclonal antibody raised against synthetic acetyl peptide of human histone H3 (acetyl K14).
A synthetic acetyl peptide corresponding to residues surrounding K14 of human histone H3.
This antibody reacts to histone H3 acetylated at Lysine 14, and is not affected by the modification of neighboring amino acids. No cross reactivity with acetylated Lysine 4, Lysine 9, Lysine 18, Lysine 23, Lysine 27, Lysine 36, or Lysine 79 in histone H3.
Protein A affinity purification
ChIP (2-10 ug/mL) ELISA (0.2-1 ug/mL) Immunocytochemistry (0.5-2 ug/mL) Western Blot (0.5-2 ug/mL) The optimal working dilution should be determined by the end user.
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
ChIP analysis of HeLa cells with Histone H3 (acetyl K14) monoclonal antibody, clone RM130 (Cat # MAB12830) under 5 ug working concentration. Real-time PCR was performed using primers specific to the gene indicated.
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cells, Lane 2: HeLa cells treated with sodium butyrate using Histone H3 (acetyl K14) monoclonal antibody, clone RM130 (Cat # MAB12830) under 0.5 ug/mL working concentration.
Immunocytochemistry staining of HeLa cells treated with sodium butyrate using Histone H3 (acetyl K14) monoclonal antibody, clone RM130 (Cat # MAB12830) (Red) under 2 ug/mL working concentration. Actin filaments were stained with fluorescein phalloidin (Green).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq