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Last updated: 2017/4/23

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GAPDH monoclonal antibody, clone RM114

  • Catalog # : MAB12829
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human GAPDH.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus region of human GAPDH.
  • Host:
  • Rabbit
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA
    Flow Cytometry
    Immunocytochemistry (1:200)
    Immunohistochemistry (1:400-1:4000)
    Immunoprecipitation (1:200)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of A431 cells using GAPDH monoclonal antibody, clone RM114 (Cat # MAB12829) at 1:1000 dilution.
  • Immunohistochemistry
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemistry staining of A431 cells with GAPDH monoclonal antibody, clone RM114 (Cat # MAB12829) (Red) at 1:200 dilution. Nuclear DNA was stained with DAPI (Blue).
  • Immunoprecipitation
  • ELISA
  • Flow Cytometry
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 2597
  • Gene Name:
  • GAPDH
  • Gene Alias:
  • G3PD,GAPD,MGC88685
  • Gene Description:
  • glyceraldehyde-3-phosphate dehydrogenase
  • Gene Summary:
  • The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000174431,OTTHUMP00000174432,aging-associated gene 9 protein,glyceraldehyde 3-phosphate dehydrogenase
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