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Last updated: 2017/7/23

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beta-actin monoclonal antibody, clone RM112

  • Catalog # : MAB12828
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human beta-actin.
  • Immunogen:
  • A synthetic peptide corresponding to N-terminus region of human beta-actin.
  • Host:
  • Rabbit
  • Specificity:
  • This antibody reacts to beta-Actin (Actin, cytoplasmic 1). No cross reactivity with other isoforms of Actin.
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA
    Flow Cytometry
    Immunocytochemistry (1:200)
    Immunohistochemistry (1:200)
    Immunoprecipitation (1:200)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of A431 cells using beta-actin monoclonal antibody, clone RM112 (Cat # MAB12828) at 1:1000 dilution.
  • Immunohistochemistry
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemistry staining of A431 cells with beta-actin monoclonal antibody, clone RM112 (Cat # MAB12828) (Red) at 1:200 dilution. Nuclear DNA was stained with DAPI (Blue).
  • Immunoprecipitation
  • ELISA
  • Flow Cytometry
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 60
  • Gene Name:
  • ACTB
  • Gene Alias:
  • PS1TP5BP1
  • Gene Description:
  • actin, beta
  • Gene Summary:
  • This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
  • Other Designations:
  • PS1TP5-binding protein 1,actin, cytoplasmic 1,beta actin,beta cytoskeletal actin
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