Rabbit monoclonal antibody raised against phosphotyrosine.
Phosphotyrosine conjugated with BSA.
This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine
Protein A affinity purification
ELISA Flow Cytometry Immunocytochemistry (1:200-1:500) Immunohistochemistry (1:200-1:500) Immunoprecipitation (1:200-1:1000) Western Blot (1:1000-1:5000) The optimal working dilution should be determined by the end user.
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of serum-starved A431 cells nontreated or treated with 100 ng/mL EGF using Phosphotyrosine monoclonal antibody, clone RM111 (Cat # MAB12826) at 1:5000 dilution.
Immunocytochemistry staining of serum-starved A431 cells (A) and serum-starved A431 cells treated with EGF (B) using Phosphotyrosine monoclonal antibody, clone RM111 (Cat # MAB12826) (Red) at 1:500 dilution. Nuclear DNA was stained with DAPI (Blue).
Immunoprecipitation analysis of Lane 1: EGE-treated A431 cell whole lysates, Lane 2: EGE-treated A431 cell whole lysates using rabbit IgG antibody; Lane 3: EGE-treated A431 cell whole lysates using Phosphotyrosine monoclonal antibody, clone RM111 (Cat # MAB12826) at 1:1000 dilution.