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Phosphothreonine monoclonal antibody, clone RM102

  • Catalog # : MAB12824
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against phosphothreonine.
  • Immunogen:
  • A mixture of phosphothreonine conjugated with BSA and phosphothreonine containing peptide.
  • Host:
  • Rabbit
  • Specificity:
  • This antibody reacts threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA
    Flow Cytometry
    Immunocytochemistry (1:100-1:500)
    Immunohistochemistry (1:100-1:500)
    Immunoprecipitation (1:100-1:500)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: serum-starved A431 cells, Lane 2: serum-starved A431 cells treated with Calyculin A/Okadaic Acid using Phosphothreonine monoclonal antibody, clone RM102 (Cat # MAB12824) at 1:2000 dilution.
  • Immunohistochemistry
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemistry staining of serum-starved A431 cells (A) and serum-starved A431 cells treated with Calyculin A/Okadaic Acid (B) using Phosphothreonine monoclonal antibody, clone RM102 (Cat # MAB12824) (Red) at 1:500 dilution. Nuclear DNA was stained with DAPI (Blue).
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation analysis of Lane 1: Calyculin A/Okadaic Acid-treated A431 cell whole lysates, Lane 2: Calyculin A/Okadaic Acid-treated A431 cell whole lysates using rabbit IgG antibody; Lane 3: Calyculin A/Okadaic Acid-treated A431 cell whole lysates using Phosphothreonine monoclonal antibody, clone RM102 (Cat # MAB12824) at 1:500 dilution.
  • ELISA
  • Flow Cytometry
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • ELISA
  • Flow Cytometry
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