Phosphothreonine monoclonal antibody, clone RM102
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: serum-starved A431 cells, Lane 2: serum-starved A431 cells treated with Calyculin A/Okadaic Acid using Phosphothreonine monoclonal antibody, clone RM102 (Cat # MAB12824) at 1:2000 dilution.
Immunocytochemistry
Immunocytochemistry staining of serum-starved A431 cells (A) and serum-starved A431 cells treated with Calyculin A/Okadaic Acid (B) using Phosphothreonine monoclonal antibody, clone RM102 (Cat # MAB12824) (Red) at 1:500 dilution. Nuclear DNA was stained with DAPI (Blue).
Immunoprecipitation
Immunoprecipitation analysis of Lane 1: Calyculin A/Okadaic Acid-treated A431 cell whole lysates, Lane 2: Calyculin A/Okadaic Acid-treated A431 cell whole lysates using rabbit IgG antibody; Lane 3: Calyculin A/Okadaic Acid-treated A431 cell whole lysates using Phosphothreonine monoclonal antibody, clone RM102 (Cat # MAB12824) at 1:500 dilution.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Phosphothreonine monoclonal antibody, clone RM102 (Cat# MAB12824) recognizes Phosphorylated threonine in peptides with different sequences. It has minimal cross- reactivity with phosphorylated serine.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against phosphothreonine.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a mixture of phosphothreonine conjugated with BSA and phosphothreonine containing peptide.
Sequence
N/A
Specificity
This antibody reacts threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry (1:100-1:500)
Immunohistochemistry (1:100-1:500)
Immunoprecipitation (1:100-1:500)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: serum-starved A431 cells, Lane 2: serum-starved A431 cells treated with Calyculin A/Okadaic Acid using Phosphothreonine monoclonal antibody, clone RM102 (Cat # MAB12824) at 1:2000 dilution.Immunohistochemistry
Immunocytochemistry
Immunocytochemistry staining of serum-starved A431 cells (A) and serum-starved A431 cells treated with Calyculin A/Okadaic Acid (B) using Phosphothreonine monoclonal antibody, clone RM102 (Cat # MAB12824) (Red) at 1:500 dilution. Nuclear DNA was stained with DAPI (Blue).Immunoprecipitation
Immunoprecipitation analysis of Lane 1: Calyculin A/Okadaic Acid-treated A431 cell whole lysates, Lane 2: Calyculin A/Okadaic Acid-treated A431 cell whole lysates using rabbit IgG antibody; Lane 3: Calyculin A/Okadaic Acid-treated A431 cell whole lysates using Phosphothreonine monoclonal antibody, clone RM102 (Cat # MAB12824) at 1:500 dilution.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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