Rabbit monoclonal antibody raised against acetylated-lysine.
Acetyllysine conjugated with BSA.
This antibody reacts to lysine-acetylated proteins. No cross reactivity with nonacetylated lysine, and lysine with other modification.
Protein A affinity purification
ELISA Flow Cytometry Immunocytochemistry (1:100-1:500) Immunohistochemistry (1:100-1:500) Immunoprecipitation (1:100-1:500) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of HeLa cells nontreated or treated with 400 nM Trichostatin A (TSA) using Acetylated-Lysine monoclonal antibody, clone RM101 (Cat # MAB12823) at 1:2000 dilution.
Immunocytochemistry staining of A431 cells (A) and A431 cells treated with Trichostatin A (TSA) (B) using Acetylated-Lysine monoclonal antibody, clone RM101 (Cat # MAB12823) (Red) at 1:500 dilution. Nuclear DNA was stained with DAPI (Blue).
Immunoprecipitation analysis of Lane 1: A431 cell whole lysates, Lane 2: TSA-treated A431 cell whole lysates; Lane 3: A431 cell whole lysates, Lane 4: TSA-treated A431 cell whole lysates using rabbit IgG antibody; Lane 5: A431 cell whole lysates, Lane 6: TSA-treated A431 cell whole lysates using Acetylated-Lysine monoclonal antibody, clone RM101 (Cat # MAB12823) at 1:500 dilution.