Acetylated-Lysine monoclonal antibody, clone RM101
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HeLa cells nontreated or treated with 400 nM Trichostatin A (TSA) using Acetylated-Lysine monoclonal antibody, clone RM101 (Cat # MAB12823) at 1:2000 dilution.
Immunocytochemistry
Immunocytochemistry staining of A431 cells (A) and A431 cells treated with Trichostatin A (TSA) (B) using Acetylated-Lysine monoclonal antibody, clone RM101 (Cat # MAB12823) (Red) at 1:500 dilution. Nuclear DNA was stained with DAPI (Blue).
Immunocytochemistry
Immunocytochemical staining of HeLa cells using Acetylated-Lysine monoclonal antibody, clone RM101 (Cat# MAB12823) (red). Actin filaments was labeled with fluorescein phalloidin (green), and nucleus stained with DAPI (blue).
Immunoprecipitation
Immunoprecipitation analysis of Lane 1: A431 cell whole lysates, Lane 2: TSA-treated A431 cell whole lysates; Lane 3: A431 cell whole lysates, Lane 4: TSA-treated A431 cell whole lysates using rabbit IgG antibody; Lane 5: A431 cell whole lysates, Lane 6: TSA-treated A431 cell whole lysates using Acetylated-Lysine monoclonal antibody, clone RM101 (Cat # MAB12823) at 1:500 dilution.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Acetylated-Lysine monoclonal antibody, clone RM101 (Cat# MAB12823) recognizes acetylated lysine in peptides with different sequences. It does not react with non-acetylated lysine.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against acetylated-lysine.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against acetyllysine conjugated with BSA.
Sequence
N/A
Specificity
This antibody reacts to lysine-acetylated proteins. No cross reactivity with nonacetylated lysine, and lysine with other modification.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry (1:100-1:500)
Immunohistochemistry (1:100-1:500)
Immunoprecipitation (1:100-1:500)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cells nontreated or treated with 400 nM Trichostatin A (TSA) using Acetylated-Lysine monoclonal antibody, clone RM101 (Cat # MAB12823) at 1:2000 dilution.Immunohistochemistry
Immunocytochemistry
Immunocytochemistry staining of A431 cells (A) and A431 cells treated with Trichostatin A (TSA) (B) using Acetylated-Lysine monoclonal antibody, clone RM101 (Cat # MAB12823) (Red) at 1:500 dilution. Nuclear DNA was stained with DAPI (Blue).Immunocytochemistry
Immunocytochemical staining of HeLa cells using Acetylated-Lysine monoclonal antibody, clone RM101 (Cat# MAB12823) (red). Actin filaments was labeled with fluorescein phalloidin (green), and nucleus stained with DAPI (blue).Immunoprecipitation
Immunoprecipitation analysis of Lane 1: A431 cell whole lysates, Lane 2: TSA-treated A431 cell whole lysates; Lane 3: A431 cell whole lysates, Lane 4: TSA-treated A431 cell whole lysates using rabbit IgG antibody; Lane 5: A431 cell whole lysates, Lane 6: TSA-treated A431 cell whole lysates using Acetylated-Lysine monoclonal antibody, clone RM101 (Cat # MAB12823) at 1:500 dilution.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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