Human IgG4 (Fc) monoclonal antibody, clone RM217
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More Files
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Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgG4 (Fc) monoclonal antibody, clone RM217 (Cat # MAB12822) at the following concentrations: 0.2 ug/mL, 0.1 ug/mL, 0.05 ug/mL, 0.025 ug/mL, 0.013 ug/mL, 0.006 ug/mL, 0.003 ug/mL. The plate was coated with 50 uL/well different amounts of human IgG4. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgG4 (Fc) monoclonal antibody, clone RM217 (Cat# MAB12822) as the capture antibody, and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an AP conjugated streptavidin.
Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgG4 (Fc) monoclonal antibody, clone RM217 (Cat# MAB12822) as the capture antibody, and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an AP conjugated streptavidin.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Human IgG4 (Fc) monoclonal antibody, clone RM217 (Cat# MAB12822) specifically reacts to human IgG4. No cross reactivity with human IgG1, IgG2, IgG3.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human IgG4 (Fc).
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to the Fc region of human IgG4.
Sequence
N/A
Reactivity
Human
Specificity
This antibody reacts to the Fc region of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA (0.05-0.2 ug/mL for detection; 0.05-0.2 ug/well for capture)
Flow Cytometry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgG4 (Fc) monoclonal antibody, clone RM217 (Cat # MAB12822) at the following concentrations: 0.2 ug/mL, 0.1 ug/mL, 0.05 ug/mL, 0.025 ug/mL, 0.013 ug/mL, 0.006 ug/mL, 0.003 ug/mL. The plate was coated with 50 uL/well different amounts of human IgG4. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgG4 (Fc) monoclonal antibody, clone RM217 (Cat# MAB12822) as the capture antibody, and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an AP conjugated streptavidin.Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgG4 (Fc) monoclonal antibody, clone RM217 (Cat# MAB12822) as the capture antibody, and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an AP conjugated streptavidin.Flow Cytometry
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Gene Info — IGHG4
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Interactome
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