Rabbit monoclonal antibody raised against synthetic peptide of human IgG4.
A synthetic peptide corresponding to the hinge region of human IgG4.
This antibody reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Protein A affinity purification
ELISA (0.05-0.2 ug/mL for detection; 0.05-0.2 ug/well for capture) Flow Cytometry Immunocytochemistry (1-10 ug/mL) Immunohistochemistry (1-10 ug/mL) Western Blot (0.5-2 ug/mL) The optimal working dilution should be determined by the end user.
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymphoid tissue with Human IgG4 monoclonal antibody, clone RM120 (Cat # MAB12821) under 5 ug/mL working concentration.
ELISA analysis of Human IgG4 monoclonal antibody, clone RM120 (Cat # MAB12821) at the following concentrations: 0.2 ug/mL, 0.1 ug/mL, 0.05 ug/mL, 0.025 ug/mL, 0.013 ug/mL, 0.006 ug/mL, 0.003 ug/mL. The plate was coated with 50 uL/well different amounts of human IgG4. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.