Human IgG3 monoclonal antibody, clone RM119
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymphoid tissue with Human IgG3 monoclonal antibody, clone RM119 (Cat # MAB12820) under 1 ug/mL working concentration.
Immunohistochemistry
Immunohistochemical staining of FFPE Tonsil tissue sections using Human IgG3 monoclonal antibody, clone RM119 (Cat# MAB12820) at a 1:2000 dilution.
Enzyme-linked Immunoabsorbent Assay
ELISA showing Human IgG3 monoclonal antibody, clone RM119 (Cat# MAB12820) does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of Cat# MAB12820 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgG3 monoclonal antibody, clone RM119 (Cat # MAB12820) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to human IgG3; no cross reactivity human IgG1, IgG2, IgG4, IgE, IgA, or IgD, mouse IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgG3 monoclonal antibody, clone RM119 (Cat# MAB12820) as the capture antibody (100ng/well), and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an HRP conjugated streptavidin.
Enzyme-linked Immunoabsorbent Assay
A titer ELISA using Human IgG3 monoclonal antibody, clone RM119 (Cat# MAB12820). The plate was coated with different amounts of human IgG3. A serial dilution of Cat# MAB12820 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
Detection of human IgG3 in monkey serum. Sandwich ELISA using Human IgG3 monoclonal antibody, clone RM119 (Cat# MAB12820) as the capture antibody, and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by a HRP conjugated streptavidin.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human IgG3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against human IgG3.
Sequence
N/A
Reactivity
Human
Specificity
This antibody reacts to the heavy chain of human IgG3. No cross reactivity with human IgG1, IgG2, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA (0.05-0.2 ug/mL for detection; 0.05-0.2 ug/well for capture)
Flow Cytometry
Immunocytochemistry (0.5-2 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymphoid tissue with Human IgG3 monoclonal antibody, clone RM119 (Cat # MAB12820) under 1 ug/mL working concentration.Immunohistochemistry
Immunohistochemical staining of FFPE Tonsil tissue sections using Human IgG3 monoclonal antibody, clone RM119 (Cat# MAB12820) at a 1:2000 dilution.Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
ELISA showing Human IgG3 monoclonal antibody, clone RM119 (Cat# MAB12820) does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of Cat# MAB12820 and a monkey IgG binding antibody (positive control) was used as the detection antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgG3 monoclonal antibody, clone RM119 (Cat # MAB12820) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to human IgG3; no cross reactivity human IgG1, IgG2, IgG4, IgE, IgA, or IgD, mouse IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgG3 monoclonal antibody, clone RM119 (Cat# MAB12820) as the capture antibody (100ng/well), and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an HRP conjugated streptavidin.Enzyme-linked Immunoabsorbent Assay
A titer ELISA using Human IgG3 monoclonal antibody, clone RM119 (Cat# MAB12820). The plate was coated with different amounts of human IgG3. A serial dilution of Cat# MAB12820 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
Detection of human IgG3 in monkey serum. Sandwich ELISA using Human IgG3 monoclonal antibody, clone RM119 (Cat# MAB12820) as the capture antibody, and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by a HRP conjugated streptavidin.Flow Cytometry
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Gene Info — IGHG3
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Interactome
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Disease
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