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Human IgG3 monoclonal antibody, clone RM119

  • Catalog # : MAB12820
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against native human IgG3.
  • Immunogen:
  • Native purified human IgG3.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • This antibody reacts to the heavy chain of human IgG3. No cross reactivity with human IgG1, IgG2, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.05-0.2 ug/mL for detection; 0.05-0.2 ug/well for capture)
    Flow Cytometry
    Immunocytochemistry (0.5-2 ug/mL)
    Immunohistochemistry (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymphoid tissue with Human IgG3 monoclonal antibody, clone RM119 (Cat # MAB12820) under 1 ug/mL working concentration.
  • Immunocytochemistry
  • ELISA
  • ELISA
  • ELISA analysis of Human IgG3 monoclonal antibody, clone RM119 (Cat # MAB12820) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to human IgG3; no cross reactivity human IgG1, IgG2, IgG4, IgE, IgA, or IgD, mouse IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
  • Flow Cytometry
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 3502
  • Gene Name:
  • IGHG3
  • Gene Alias:
  • DKFZp686H11213,FLJ39988,FLJ40036,FLJ40253,FLJ40587,FLJ40789,FLJ40834,IgG3,MGC45809
  • Gene Description:
  • immunoglobulin heavy constant gamma 3 (G3m marker)
  • Other Designations:
  • -
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