Human IgG2 monoclonal antibody, clone RM118
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More Files
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Enzyme-linked Immunoabsorbent Assay
ELISA showing Human IgG2 monoclonal antibody, clone RM118 (Cat# MAB12819) does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of Cat# MAB12819 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgG2 monoclonal antibody, clone RM118 (Cat # MAB12819) at the following concentrations: 0.1 ug/mL, 0.033 ug/mL, 0.0111 ug/mL, 0.0037 ug/mL, 0.0012 ug/mL, 0.0004 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to human IgG2; no cross reactivity human IgG1, IgG3, IgG4, IgE, IgA, or IgD, mouse IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
A titer ELISA using Human IgG2 monoclonal antibody, clone RM118 (Cat# MAB12819). The plate was coated with different amounts of human IgG2. A serial dilution of Cat# MAB12819 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
Detection of human IgG2 in monkey serum. Sandwich ELISA using Human IgG2 monoclonal antibody, clone RM118 (Cat# MAB12819) as the capture antibody, and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by a HRP conjugated streptavidin.
Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgG2 monoclonal antibody, clone RM118 (Cat# MAB12819) as the capture antibody, and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an AP conjugated streptavidin.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human IgG2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against human IgG2.
Sequence
N/A
Reactivity
Human
Specificity
This antibody reacts to the heavy chain of human IgG2. No cross reactivity with human IgG1, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA (0.05-0.2 ug/mL for detection; 0.05-0.2 ug/well for capture)
Flow Cytometry
Immunocytochemistry (0.5-2 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry
Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
ELISA showing Human IgG2 monoclonal antibody, clone RM118 (Cat# MAB12819) does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of Cat# MAB12819 and a monkey IgG binding antibody (positive control) was used as the detection antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgG2 monoclonal antibody, clone RM118 (Cat # MAB12819) at the following concentrations: 0.1 ug/mL, 0.033 ug/mL, 0.0111 ug/mL, 0.0037 ug/mL, 0.0012 ug/mL, 0.0004 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to human IgG2; no cross reactivity human IgG1, IgG3, IgG4, IgE, IgA, or IgD, mouse IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
A titer ELISA using Human IgG2 monoclonal antibody, clone RM118 (Cat# MAB12819). The plate was coated with different amounts of human IgG2. A serial dilution of Cat# MAB12819 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
Detection of human IgG2 in monkey serum. Sandwich ELISA using Human IgG2 monoclonal antibody, clone RM118 (Cat# MAB12819) as the capture antibody, and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by a HRP conjugated streptavidin.Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgG2 monoclonal antibody, clone RM118 (Cat# MAB12819) as the capture antibody, and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an AP conjugated streptavidin.Flow Cytometry
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Gene Info — IGHG2
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Interactome
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Disease
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