Rabbit monoclonal antibody raised against synthetic peptide of human IgG1.
A synthetic peptide corresponding to the hinge region of human IgG1.
This antibody reacts to the heavy chain of human IgG1. No cross reactivity with human IgG2, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Protein A affinity purification
ELISA (0.05-0.2 ug/mL for detection; 0.05-0.2 ug/well for capture) Flow Cytometry Immunocytochemistry (0.5-2 ug/mL) Immunohistochemistry (0.5-2 ug/mL) The optimal working dilution should be determined by the end user.
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
ELISA analysis of Human IgG1 monoclonal antibody, clone RM117 (Cat # MAB12818) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to human IgG1; no cross reactivity human IgG2, IgG3, IgG4, IgE, IgA, or IgD, mouse IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.