Human IgG1 monoclonal antibody, clone RM117
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Specifications
Product Description
Rabbit recombinant monoclonal antibody raised against of human IgG1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to the hinge region of human IgG1.
Sequence
N/A
Reactivity
Human
Specificity
This antibody reacts to the heavy chain of human IgG1. No cross reactivity with human IgG2, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA (0.05-0.2 ug/mL for detection; 0.05-0.2 ug/well for capture)
Flow Cytometry
Immunocytochemistry (0.5-2 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry
Immunohistochemistry
Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG1 antibody Human IgG1 monoclonal antibody, clone RM117 (Cat# MAB12818) at a 1:15,000 dilution.Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgG1 monoclonal antibody, clone RM117 (Cat # MAB12818) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to human IgG1; no cross reactivity human IgG2, IgG3, IgG4, IgE, IgA, or IgD, mouse IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Flow Cytometry
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Gene Info — IGHG1
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Interactomes
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Diseases
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