Human IgD monoclonal antibody, clone RM123
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymphoid tissue with Human IgD monoclonal antibody, clone RM123 (Cat # MAB12816) under 1 ug/mL working concentration.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgD monoclonal antibody, clone RM123 (Cat # MAB12816) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to human IgD kappa; no cross reactivity human IgG, IgM, IgA, or IgE. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
A titer ELISA using Human IgD monoclonal antibody, clone RM123 (Cat# MAB12816). The plate was coated with different amounts of human IgD (from plasma). A serial dilution of Cat# MAB12816 was used as the primary antibody. An alkaline phosphatase conjugated anti- rabbit IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgD monoclonal antibody, clone RM123 (Cat# MAB12816) as the capture antibody (100ng/well), and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human IgD.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against human IgD.
Sequence
N/A
Reactivity
Human
Specificity
This antibody reacts to human IgD. No cross reactivity with human IgG, IgM, IgE, or IgA.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA (0.01-0.1 ug/mLfor detection; 0.025-0.2 ug/well for capture)
Flow Cytometry
Immunocytochemistry (0.5-2 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymphoid tissue with Human IgD monoclonal antibody, clone RM123 (Cat # MAB12816) under 1 ug/mL working concentration.Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgD monoclonal antibody, clone RM123 (Cat # MAB12816) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to human IgD kappa; no cross reactivity human IgG, IgM, IgA, or IgE. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
A titer ELISA using Human IgD monoclonal antibody, clone RM123 (Cat# MAB12816). The plate was coated with different amounts of human IgD (from plasma). A serial dilution of Cat# MAB12816 was used as the primary antibody. An alkaline phosphatase conjugated anti- rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgD monoclonal antibody, clone RM123 (Cat# MAB12816) as the capture antibody (100ng/well), and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.Flow Cytometry
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Gene Info — IGHD
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Interactome
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