Rabbit monoclonal antibody raised against native human IgA2.
Native purified IgA2 from human plasma.
This antibody reacts to human IgA2. No cross reactivity with human IgA1, IgG, IgM, IgD, or IgE.
Protein A affinity purification
ELISA (0.05-0.2 ug/mL for detection; 0.05-0.2 ug/well for capture) Flow Cytometry Immunocytochemistry (0.5-2 ug/mL) Immunohistochemistry (0.1-1 ug/mL) The optimal working dilution should be determined by the end user.
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil tissue with Human IgA2 monoclonal antibody, clone RM125 (Cat # MAB12815) under 1 ug/mL working concentration.
ELISA analysis of Human IgA2 monoclonal antibody, clone RM125 (Cat # MAB12815) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to human IgA2; no cross reactivity human IgG, IgM, IgD, or IgE. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.